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Hydrogen Peroxide Induced Activation of Gene Expression in Mammalian Cells using Boronate Estrone Derivatives
Author(s) -
Govan Jeane M.,
McIver Andrew L.,
Riggsbee Chad,
Deiters Alexander
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201203222
Subject(s) - estrone , hydrogen peroxide , gene , gene expression , chemistry , estrogen , estrogen receptor , combinatorial chemistry , computational biology , biochemistry , biology , genetics , hormone , cancer , breast cancer
Keeping the boron out of the ER : A genetic switch was engineered that activates gene expression in the presence of H 2 O 2 (see scheme). The use of a boronate group on an estrone molecule allows for activation of gene expression through binding of the estrogen receptor only when the boron group is oxidized by H 2 O 2 . This sensor is highly sensitive and specific for H 2 O 2 .