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A Tunable Ratiometric pH Sensor Based on Carbon Nanodots for the Quantitative Measurement of the Intracellular pH of Whole Cells
Author(s) -
Shi Wen,
Li Xiaohua,
Ma Huimin
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201202533
Subject(s) - nanodot , hela , intracellular , fluorescence , nanosensor , carbon fibers , biocompatibility , chemistry , nanotechnology , intracellular ph , biophysics , computer science , materials science , analytical chemistry (journal) , biochemistry , biology , chromatography , physics , optics , organic chemistry , cell , composite number , algorithm
The whole picture : Carbon nanodots labeled with two fluorescent dyes have been developed as a tunable ratiometric pH sensor to measure intracellular pH. The nanosensor shows good biocompatibility and cellular dispersibility. Quantitative determinations on intact HeLa cells and pH fluctuations associated with oxidative stress were performed.