Premium
GRecon: A Method for the Lipid Reconstitution of Membrane Proteins
Author(s) -
Althoff Thorsten,
Davies Karen M.,
Schulze Sabrina,
Joos Friederike,
Kühlbrandt Werner
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201202094
Subject(s) - membrane protein , liposome , lipid bilayer , membrane , chemistry , peripheral membrane protein , protein–lipid interaction , biological membrane , biochemistry , integral membrane protein
Density gradients with increasing concentrations of cyclodextrin and detergent‐destabilized liposomes are loaded with detergent‐solubilized membrane proteins in a new approach for reconstituting membrane proteins. During centrifugation, the detergent is gradually replaced by the lipid. This gentle method separates proteoliposomes from empty liposomes and is particularly suitable for the lipid reconstitution of large, fragile membrane proteins.