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DNA Polyfluorophores for Real‐Time Multicolor Tracking of Dynamic Biological Systems
Author(s) -
Wang Shenliang,
Guo Jia,
Ono Toshikazu,
Kool Eric T.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201201928
Subject(s) - multispectral image , computer science , tracking (education) , set (abstract data type) , scheme (mathematics) , information retrieval , artificial intelligence , programming language , psychology , mathematical analysis , pedagogy , mathematics
Dye‐ing to live : Spectral limitations of common organic dyes make it difficult or impossible to visualize and follow multiple biological components in rapidly moving systems. The development of a multispectral set of improved DNA‐scaffolded fluorophores is described. Their use in multicolor cellular imaging (see scheme) and in tracking of biological motions on the subsecond timescale is demonstrated.