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Direct Visualization of the Movement of a Single T7 RNA Polymerase and Transcription on a DNA Nanostructure
Author(s) -
Endo Masayuki,
Tatsumi Koichi,
Terushima Kosuke,
Katsuda Yousuke,
Hidaka Kumi,
Harada Yoshie,
Sugiyama Hiroshi
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201201890
Subject(s) - t7 rna polymerase , transcription (linguistics) , dna , biotinylation , dna origami , polymerase , rna , rna polymerase , rna polymerase ii , chemistry , biophysics , microbiology and biotechnology , transcription bubble , rna dependent rna polymerase , promoter , nanotechnology , biology , biochemistry , gene , gene expression , materials science , bacteriophage , linguistics , philosophy , escherichia coli
Snapshots of transcription : Movement of a single molecule of T7 RNA polymerase (RNAP) along a double‐stranded DNA (dsDNA) template attached to a DNA origami platform (green, see scheme) was recorded using high‐speed AFM. A one kilobase template dsDNA containing the T7 promoter was used to record AFM images of transcription of the template dsDNA by RNAP and the resulting biotinylated RNA product was detected by streptavidin‐labeling.