Premium
Polyspermine Imidazole‐4,5‐imine, a Chemically Dynamic and Biologically Responsive Carrier System for Intracellular Delivery of siRNA
Author(s) -
Duan Shiyue,
Yuan Weien,
Wu Fei,
Jin Tuo
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201201793
Subject(s) - endosome , gene silencing , imidazole , chemistry , intracellular , imine , small interfering rna , cytotoxicity , rna interference , transfection , microbiology and biotechnology , biophysics , biochemistry , in vitro , biology , rna , gene , catalysis
Silence please! Polyspermine imidazole‐4,5‐imine (blue lines) was formed by condensing spermine with bisformaldehyde imidazole through a pH‐responsive linkage. This polymer was used to condense siRNAs into nanoparticles (see scheme) and studied for delivery into cells and release from endosomes. Cellular and in vivo assays indicated that this siRNA carrier was efficient in silencing target genes with negligible cytotoxicity.