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Strong and Selective Binding of Amiloride to an Abasic Site in RNA Duplexes: Thermodynamic Characterization and MicroRNA Detection
Author(s) -
Sato Yusuke,
Ichihashi Toshiki,
Nishizawa Seiichi,
Teramae Norio
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201201790
Subject(s) - duplex (building) , rna , amiloride , microrna , binding site , chemistry , computational biology , fluorescence , dna , biophysics , combinatorial chemistry , biochemistry , biology , gene , physics , sodium , organic chemistry , quantum mechanics
Firmly tied : The binding affinity of amiloride for an abasic (AP) site‐containing RNA duplex (see picture) is two orders of magnitude superior to the affinity of the corresponding AP site‐containing DNA duplex. The observed high binding affinity for the RNA duplex arises from a favorable enthalpy gain. The binding‐induced fluorescence response of amiloride is applicable to microRNA detection.