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Protein Tango: The Toolbox to Capture Interacting Partners
Author(s) -
Rutkowska Anna,
Schultz Carsten
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201201717
Subject(s) - toolbox , context (archaeology) , protein function , identification (biology) , protein–protein interaction , function (biology) , computational biology , living cell , chemistry , nanotechnology , computer science , microbiology and biotechnology , biology , biochemistry , materials science , gene , paleontology , botany , programming language
The evaluation of protein function in the context of the whole cell is crucial for understanding of living systems. In this context, the identification and modulation of protein–protein interactions in and outside cells is of ample importance. Several methods have been developed in the past years to detect and/or actively induce protein–protein interactions in living cells. As a result, tools are now available to manipulate intracellular events by reversible or irreversible cross‐linking of proteins in a specific manner. These techniques open many new doors and enable the dissection of complicated protein networks. Herein we describe which cross‐linkers and inducers of dimerization are out there and how to make use of this great toolbox.