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(IscS‐IscU) 2 Complex Structures Provide Insights into Fe 2 S 2 Biogenesis and Transfer
Author(s) -
Marii Elodie N.,
de Oliveira Jaim S.,
Nicolet Yvain,
Raulfs Estella C.,
Amara Patricia,
Dean Dennis R.,
FontecillaCamps Juan C.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201201708
Subject(s) - biogenesis , chemistry , computational biology , biology , biochemistry , gene
The center of attention : IscS cysteine desulfurases and IscU scaffolds are involved in biological iron–sulfur cluster assembly. The X‐ray structure of an anaerobically produced, mutated (Fe 2 S 2 ‐(IscS‐IscU D35A )) 2 complex reveals a cluster coordinated by three IscU cysteines and the IscS active cysteine (see picture). In air‐exposed crystals the cluster is oxidized to an Fe 2 S–S center; D35 is essential for complex dissociation.