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Single‐Molecule Approach to DNA Minor‐Groove Association Dynamics
Author(s) -
Bordello Jorge,
Sánchez Mateo I.,
Vázquez M. Eugenio,
Mascareñas José L.,
AlSoufi Wajih,
Novo Mercedes
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201201099
Subject(s) - minor groove , groove (engineering) , association (psychology) , minor (academic) , event (particle physics) , dynamics (music) , dna , computer science , information retrieval , computational biology , chemistry , physics , genetics , biology , engineering , psychology , philosophy , humanities , acoustics , psychotherapist , mechanical engineering , quantum mechanics
Getting in the groove : Fluorescence correlation spectroscopy reveals that the dynamics of the association process of the bisbenzamidine minor‐groove binder BBA‐OG (blue with green star, see scheme) to dsDNA is not controlled by diffusion, but by the insertion of the binder into the groove at the specific site (red), as shown by the rate constants for each step of the binding event.