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Hyperpolarized Binding Pocket Nuclear Overhauser Effect for Determination of Competitive Ligand Binding
Author(s) -
Lee Youngbok,
Zeng Haifeng,
Mazur Adam,
Wegstroth Melanie,
Carlomagno Teresa,
Reese Marcel,
Lee Donghan,
Becker Stefan,
Griesinger Christian,
Hilty Christian
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201201003
Subject(s) - chemistry , ligand (biochemistry) , nuclear overhauser effect , polarization (electrochemistry) , binding site , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , stereochemistry , physics , biochemistry , receptor
Protein‐mediated polarization transfer : Ligands L1 and L2 that competitively bind to a protein are subject to indirect spin‐polarization transfer through the binding site of the protein. If protons H L1 of one ligand are hyperpolarized by dynamic nuclear polarization (DNP, see picture), signal intensities in the NMR spectrum of the second ligand become enhanced. The relative build‐up of signal of the second ligand yields information on its binding epitope.