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Significantly Improved Analytical Sensitivity of Lateral Flow Immunoassays by Using Thermal Contrast
Author(s) -
Qin Zhenpeng,
Chan Warren C. W.,
Boulware David R.,
Akkin Taner,
Butler Elissa K.,
Bischof John C.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201200997
Subject(s) - sensitivity (control systems) , materials science , contrast (vision) , thermal , nanoparticle , colloidal gold , flow (mathematics) , nanotechnology , biomedical engineering , optics , medicine , mathematics , electronic engineering , physics , engineering , geometry , meteorology
Heat beyond visual : The thermal contrast from the heating of gold nanoparticles upon laser stimulation can improve the analytical sensitivity of lateral flow assays (LFAs; see picture). A 32‐fold improvement in sensitivity of an approved LFA for cryptococcal antigen (purple diamond) was shown, with the potential for further improvement by optimizing the backing material and the properties of the antibody‐coated nanoparticles (red circle with blue Y).