Premium
Triple Bioorthogonal Ligation Strategy for Simultaneous Labeling of Multiple Enzymatic Activities
Author(s) -
Willems Lianne I.,
Li Nan,
Florea Bogdan I.,
Ruben Mark,
van der Marel Gijsbert A.,
Overkleeft Herman S.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201200923
Subject(s) - bioorthogonal chemistry , ligation , cycloaddition , orthogonality , computer science , chemistry , combinatorial chemistry , click chemistry , catalysis , biochemistry , biology , microbiology and biotechnology , mathematics , geometry
Three at the same time : A ligation strategy combining tetrazine–norbornene cycloaddition, Staudinger–Bertozzi ligation, and copper(I)‐catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.