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Triple‐Color Super‐Resolution Imaging of Live Cells: Resolving Submicroscopic Receptor Organization in the Plasma Membrane
Author(s) -
Wilmes Stephan,
Staufenbiel Markus,
Liße Domenik,
Richter Christian P.,
Beutel Oliver,
Busch Karin B.,
Hess Samuel T.,
Piehler Jacob
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201200853
Subject(s) - context (archaeology) , membrane , resolution (logic) , superresolution , live cell imaging , g protein coupled receptor , chemistry , high resolution , nanotechnology , biophysics , receptor , computer science , biology , artificial intelligence , materials science , biochemistry , image (mathematics) , cell , history , paleontology , archaeology
In living color : Efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super‐resolution imaging in live cells is described. The dynamics of cellular nanostructures at the plasma membrane were monitored with a time resolution of a few seconds. In combination with dual‐color FPALM imaging, submicroscopic receptor organization within the context of the membrane skeleton was resolved (see figure).