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A Sedimented Sample of a 59 kDa Dodecameric Helicase Yields High‐Resolution Solid‐State NMR Spectra
Author(s) -
Gardiennet Carole,
Schütz Anne K.,
Hunkeler Andreas,
Kunert Britta,
Terradot Laurent,
Böckmann Anja,
Meier Beat H.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201200779
Subject(s) - yield (engineering) , nmr spectra database , dnab helicase , spectral line , crystallography , solid state , chemistry , sample (material) , resolution (logic) , high resolution , helicase , analytical chemistry (journal) , materials science , computer science , physics , chromatography , biochemistry , geology , metallurgy , rna , remote sensing , astronomy , artificial intelligence , gene
Crystal clear : Preparing solid‐state NMR samples that yield high‐resolution spectra displaying high sensitivity is time‐consuming and complicated. A sample of the 59 kDa protein DnaB, prepared simply by preparative centrifugation, provides spectra that are as good as the ones from carefully grown microcrystals.