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Fully Integrated Thermoplastic Genosensor for the Highly Sensitive Detection and Identification of Multi‐Drug‐Resistant Tuberculosis
Author(s) -
Wang Hong,
Chen HuiWen,
Hupert Mateusz L.,
Chen PinChuan,
Datta Proyag,
Pittman Tana L.,
Goettert Jost,
Murphy Michael C.,
Williams Diana,
Barany Francis,
Soper Steven A.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201200732
Subject(s) - tuberculosis , mycobacterium tuberculosis , medicine , nucleic acid amplification tests , drug resistance , polymerase chain reaction , virology , isoniazid , ligase chain reaction , bedaquiline , microbiology and biotechnology , biology , pathology , multiplex polymerase chain reaction , genetics , chlamydia trachomatis , gene
A modular system for the reporting of drug‐resistant strains of Mycobacterium tuberculosis (Mtb) in less than 30 min with full process automation was developed (see picture; MDR‐TB=multi‐drug‐resistant tuberculosis). The fluidic cartridge uses a polymer chain reaction (PCR)/ligase detection reaction (LDR)/universal array assay that can detect, with high reliability, resistant strains that are a minority (less than 1 %) from a mixed population.