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Single‐Molecule FRET Reveals the Folding Dynamics of the Human Telomerase RNA Pseudoknot Domain
Author(s) -
Hengesbach Martin,
Kim NakKyoon,
Feigon Juli,
Stone Michael D.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201200526
Subject(s) - pseudoknot , single molecule fret , förster resonance energy transfer , telomerase , computational biology , folding (dsp implementation) , rna , chemistry , computer science , biophysics , physics , biology , gene , biochemistry , quantum mechanics , engineering , electrical engineering , fluorescence
Do knot FRET : Single‐molecule FRET was used to analyze the folding of human telomerase RNA (see scheme) and allowed discrimination between the correctly folded (left) and misfolded (right) structures. Analyzing the effects of various mutations showed that base pairing, triplex formation, and Mg 2+ ion binding act synergistically in the folding of the pseudoknot structure in a functional hTR construct.