Premium
Spatially Resolved Monitoring of Neutrophil Elastase Activity with Ratiometric Fluorescent Reporters
Author(s) -
Gehrig Stefanie,
Mall Marcus A.,
Schultz Carsten
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201109226
Subject(s) - neutrophil elastase , elastase , fluorescence , chemistry , reporter gene , inflammation , computational biology , biochemistry , biology , immunology , enzyme , gene , gene expression , physics , quantum mechanics
Finding NEmo : In a mouse model for lung inflammation, the lipidated peptide‐based ratiometric fluorescent reporter NEmo‐2 indicates that neutrophil elastase (NE) activity is mainly associated with the surface of neutrophils (see scheme), whereas a soluble reporter variant showed no activity in the lung fluid, likely because of the abundance of antiproteases. Targeting the localization of NE activity has great potential for the development of improved antiinflammatory drugs.