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Ag I Ion Mediated Formation of a C–A Mispair by DNA Polymerases
Author(s) -
Funai Tatsuya,
Miyazaki Yuki,
Aotani Megumi,
Yamaguchi Eriko,
Nakagawa Osamu,
Wada Shunichi,
Torigoe Hidetaka,
Ono Akira,
Urata Hidehito
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201109191
Subject(s) - dna polymerase , polymerase , dna , chemistry , deoxyadenosine , base pair , cytosine , stereochemistry , crystallography , biochemistry
Silver turns up the A–C : In the presence of Ag I ions, a DNA polymerase incorporated deoxyadenosine (from dATP) at the site opposite cytosine in the template strand to afford the full‐length product (see scheme), meaning that DNA polymerases prefer a C‐Ag I ‐A base pair to the more thermodynamically stable C‐Ag I ‐C base pair.