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Bound Cations Significantly Stabilize the Structure of Multiprotein Complexes in the Gas Phase
Author(s) -
Han Linjie,
Hyung SukJoon,
Ruotolo Brandon T.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201109127
Subject(s) - gas phase , chemistry , mass spectrometry , molecule , phase (matter) , multiprotein complex , protein structure , crystallography , chemical physics , chromatography , biochemistry , organic chemistry , gene
Positively a gas : Protein structure determination using mass spectrometry can be assisted by the addition of stabilizing cations to the gas‐phase molecule. Densely charged cations (see scheme, green) are found to stabilize protein tertiary structure for accurate mass determination of homo‐ or heterogeneous protein complexes. The cations remain tightly bound to the protein throughout the analysis, allowing the protein to remain in a folded state.