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RNA‐Mediated Gene Assembly from DNA Arrays
Author(s) -
Wu ChengHsien,
Lockett Matthew R.,
Smith Lloyd M.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201109058
Subject(s) - oligonucleotide , rna , microbiology and biotechnology , dna , reverse transcriptase , t7 rna polymerase , rna dependent rna polymerase , polymerase , gene , chemistry , biology , genetics , escherichia coli , bacteriophage
Gene Genie : RNA‐mediated gene assembly is possible in a single day from oligonucleotide sequences on a DNA array. A T7 promoter is appended to each surface‐bound oligonucleotide and many RNA copies of each are then produced with T7 RNA polymerase (see Figure). These RNA molecules self‐assemble into the desired full‐length transcript by hybridization and ligation, which is then converted into double‐stranded DNA by reverse transcriptase polymerase chain reaction.