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Consecutive Signal Amplification for DNA Detection Based on De Novo Fluorophore Synthesis and Host–Guest Chemistry
Author(s) -
Chen XiaoHua,
Roloff Alexander,
Seitz Oliver
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201108845
Subject(s) - dna , chemistry , combinatorial chemistry , wittig reaction , benzaldehyde , fluorophore , computational biology , computer science , stereochemistry , fluorescence , biochemistry , biology , physics , quantum mechanics , catalysis
Two is more than one : A method has been developed in which a DNA template triggers a Wittig reaction that leads to transfer of a benzylidene group from a DNA‐linked phosphonium salt to a DNA‐linked benzaldehyde. A synthetic receptor such as α‐cyclodextrin then encapsulates the newly formed stilbene and triggers further signal increases.