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Cover Picture: Dynamics and Multiple Stable Binding Modes of DNA Intercalators Revealed by Single‐Molecule Force Spectroscopy (Angew. Chem. Int. Ed. 8/2012)
Author(s) -
Paik D. Hern,
Perkins Thomas T.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201108162
Subject(s) - intercalation (chemistry) , dna , molecule , spectroscopy , chemistry , cover (algebra) , molecular dynamics , force spectroscopy , crystallography , biophysics , chemical physics , computational chemistry , physics , biochemistry , biology , inorganic chemistry , organic chemistry , quantum mechanics , mechanical engineering , engineering
Intercalation of YOYO a bisintercalating DNA dye, was studied by using single‐molecule force spectroscopy. In their Communication on page 1811 ff., D. H. Paik and T. T. Perkins show that force‐enhanced intercalation, traditionally ascribed to the dye in solution binding to the DNA, can occur from a reservoir of bound dye that remained out of equilibrium with the free dye for long periods of time (>2 hours). Hence, binding/unbinding and intercalation/de‐intercalation are distinct processes that can occur on very different time scales.