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Synthesis of Heterobifunctional Protein Fusions Using Copper‐Free Click Chemistry and the Aldehyde Tag
Author(s) -
Hudak Jason E.,
Barfield Robyn M.,
de Hart Gregory W.,
Grob Patricia,
Nogales Eva,
Bertozzi Carolyn R.,
Rabuka David
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201108130
Subject(s) - click chemistry , aldehyde , oxime , chemistry , residue (chemistry) , combinatorial chemistry , triazole , copper , biochemistry , organic chemistry , catalysis
Clicking into place : Cu‐free click chemistry is combined with the aldehyde tag protein modification strategy to produce heterobifunctional protein fusions. This method relies on linkers that utilize orthogonal triazole and oxime chemistries (see scheme) to expand on conjugation with the fGly residue and enables site‐specific protein conjugation to full‐length human antibodies.