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Click‐Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core
Author(s) -
Dumont Audrey,
Malleron Annie,
Awwad Monzer,
Dukan Sam,
Vauzeilles Boris
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201108127
Subject(s) - bioorthogonal chemistry , click chemistry , membrane , bacteria , chemistry , biochemistry , core (optical fiber) , computer science , lipopolysaccharide , combinatorial chemistry , computational biology , biology , genetics , telecommunications , endocrinology
Clicking bugs : Metabolically active Gram‐negative bacteria can specifically incorporate an azide‐modified analogue of 3‐deoxy‐ D ‐manno‐octulosonic acid ( 1 ; see scheme) into the lipopolysaccharides on their membranes. This process adds a bioorthogonal chemical reporter onto the cell surface and allows the rapid fluorescent labeling of viable cells by click chemistry.