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The Last Step of Kanamycin Biosynthesis: Unique Deamination Reaction Catalyzed by the α‐Ketoglutarate‐Dependent Nonheme Iron Dioxygenase KanJ and the NADPH‐Dependent Reductase KanK
Author(s) -
Sucipto Hilda,
Kudo Fumitaka,
Eguchi Tadashi
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201108122
Subject(s) - dioxygenase , deamination , kanamycin , chemistry , biosynthesis , enzyme , biochemistry , heterologous , heterologous expression , reductase , stereochemistry , gene , recombinant dna
Mystery solved : Using heterologous expression, the activities of two enzymes exclusively belonging to the kanamycin biosynthetic pathway have been identified in vitro. A distinctive reaction mechanism (see scheme) to produce kanamycin is proposed and the previously unknown catalytic deamination activity of KanJ dioxygenase is uncovered.