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A Fatigue‐Resistant Photoswitchable Fluorescent Protein for Optical Nanoscopy
Author(s) -
Nienhaus G. Ulrich
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201108036
Subject(s) - fluorescence , fluorescent protein , 3d optical data storage , materials science , optoelectronics , nanotechnology , biophysics , optics , chemistry , green fluorescent protein , physics , biology , biochemistry , gene
Like the battery bunny : The novel photoswitchable fluorescent protein rsEGFP can be cycled between its fluorescent and nonfluorescent states more than a thousand times and is, therefore, a superb marker for high‐resolution RESOLFT imaging (RESOLFT=reversible saturable optical fluorescence transition) and data storage applications.