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In Vitro and In Vivo Uncaging and Bioluminescence Imaging by Using Photocaged Upconversion Nanoparticles
Author(s) -
Yang Yanmei,
Shao Qing,
Deng Renren,
Wang Chao,
Teng Xue,
Cheng Kai,
Cheng Zhen,
Huang Ling,
Liu Zhuang,
Liu Xiaogang,
Xing Bengang
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201107919
Subject(s) - photon upconversion , in vivo , bioluminescence , in vitro , bioluminescence imaging , nanoparticle , fluorescence , nanotechnology , luciferin , biophysics , chemistry , computer science , luciferase , materials science , luminescence , optoelectronics , biochemistry , optics , biology , transfection , physics , gene , microbiology and biotechnology
Trading up : A bioimaging system that is based on caged D ‐luciferin/upconversion nanoparticle conjugate has been developed. The nanoparticles upconvert near‐infrared light into UV light, which triggers the photorelease of D ‐luciferin (see scheme) and leads to enhanced fluorescence and bioluminescence signals in vitro and in vivo. The use of near‐infrared light enables deep penetration into tissue in vivo with minimum cellular damage.