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Development of an Amine Dehydrogenase for Synthesis of Chiral Amines
Author(s) -
Abrahamson Michael J.,
VázquezFigueroa Eduardo,
Woodall Nicholas B.,
Moore Jeffrey C.,
Bommarius Andreas S.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201107813
Subject(s) - amine gas treating , enantioselective synthesis , dehydrogenase , ketone , chemistry , enzyme , organic chemistry , biochemistry , stereochemistry , catalysis
A leucine dehydrogenase has been successfully altered through several rounds of protein engineering to an enantioselective amine dehydrogenase. Instead of the wild‐type α‐keto acid, the new amine dehydrogenase now accepts the analogous ketone, methyl isobutyl ketone (MIBK), which corresponds to exchange of the carboxy group by a methyl group to produce chiral ( R )‐1,3‐dimethylbutylamine.