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Enzymatically Incorporated Genomic Tags for Optical Mapping of DNA‐Binding Proteins
Author(s) -
Kim Soohong,
Gottfried Anna,
Lin Ron R.,
Dertinger Thomas,
Kim Andrew S.,
Chung Sangyoon,
Colyer Ryan A.,
Weinhold Elmar,
Weiss Shimon,
Ebenstein Yuval
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201107714
Subject(s) - barcode , dna , computational biology , genomic dna , computer science , genome , genetics , biology , information retrieval , gene , operating system
All smiles : Sequence‐specific methyltransferase‐induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome (see picture). The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single‐molecule level.