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Ratiometric Molecular Sensor for Monitoring Oxygen Levels in Living Cells
Author(s) -
Yoshihara Toshitada,
Yamaguchi Yuji,
Hosaka Masahiro,
Takeuchi Toshiyuki,
Tobita Seiji
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201107557
Subject(s) - fluorophore , phosphorescence , oxygen sensor , iridium , fluorescence , oxygen , phosphor , coumarin , photochemistry , molecular oxygen , chemistry , computer science , combinatorial chemistry , optoelectronics , materials science , organic chemistry , optics , physics , catalysis
A ratiometric oxygen sensor (C343‐Pro 4 ‐BTP) that uses coumarin 343 (blue in picture) as an oxygen‐insensitive fluorophore and an iridium complex (BTP, red in picture) as an oxygen‐sensitive phosphor can be used to determine the oxygen concentration in solution and in living cells, which was demonstrated by ratiometric measurements of the blue coumarin 343 fluorescence and the red BTP phosphorescence in C343‐Pro 4 ‐BTP (see picture).