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Genetically Encoded Photocrosslinkers as Molecular Probes To Study G‐Protein‐Coupled Receptors (GPCRs)
Author(s) -
BeckSickinger Annette G.,
Budisa Nediljko
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201107211
Subject(s) - g protein coupled receptor , receptor , computational biology , genetic code , amino acid , biology , chemistry , biochemistry
The genetic code was expanded with orthogonal pairs to introduce photoactivatable amino acids into G‐protein‐coupled receptors (GPCRs) in a noninvasive manner. In this way the receptor surface could be mapped by searching for specific ligand interaction sites and the complex dynamics could be studied. This method is also useful for probing the structure of GPCR complexes in living cells.