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Triggered Gene Expression in Fed‐Vesicle Microreactors with a Multifunctional Membrane
Author(s) -
Nourian Zohreh,
Roelofsen Wouter,
Danelon Christophe
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201107123
Subject(s) - green fluorescent protein , vesicle , microreactor , fluorescence microscope , membrane , gene , fluorescence , chemistry , gene expression , microbiology and biotechnology , nanotechnology , computational biology , biochemistry , biology , materials science , physics , optics , catalysis
A minimal gene expression machinery has been encapsulated within large lipid vesicles that are immobilized on a microscope coverslip for fluorescence imaging (see picture). The engineered vesicle membrane acts as an exchange platform that enables the uptake of all necessary nutrients and tRNAs supplied in the outside environment, which initiates the internal synthesis of green fluorescent proteins (GFP) from a DNA template. Red: membrane dye, green: synthesized GFP.