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Phosphopeptide‐Dependent Labeling of 14 – 3 – 3 ζ Proteins by Fusicoccin‐Based Fluorescent Probes
Author(s) -
Takahashi Michiko,
Kawamura Akie,
Kato Nobuo,
Nishi Tsuyoshi,
Hamachi Itaru,
Ohkanda Junko
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201106995
Subject(s) - phosphopeptide , fusicoccin , fluorescence , fluorescent labelling , chemistry , computational biology , biochemistry , combinatorial chemistry , biology , enzyme , phosphorylation , physics , atpase , quantum mechanics
Fluorescent combination : Cell‐penetrating probes derived from the diterpene fusicoccin can form ternary complexes with 14–3–3 proteins and phosphopeptide ligands, whereupon the probes site‐specifically attach a fluorescent tag onto the surface of the 14–3–3 proteins.