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Site‐Specific Conjugation of ScFvs Antibodies to Nanoparticles by Bioorthogonal Strain‐Promoted Alkyne–Nitrone Cycloaddition
Author(s) -
Colombo Miriam,
Sommaruga Silvia,
Mazzucchelli Serena,
Polito Laura,
Verderio Paolo,
Galeffi Patrizia,
Corsi Fabio,
Tortora Paolo,
Prosperi Davide
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201106775
Subject(s) - bioorthogonal chemistry , alkyne , chemistry , conjugated system , cycloaddition , nitrone , strain (injury) , antibody , mutant , click chemistry , combinatorial chemistry , biochemistry , biology , polymer , organic chemistry , gene , immunology , anatomy , catalysis
Particularly suitable : An N‐terminal serine mutant of anti‐HER2 scFv antibody was conjugated to polymer‐coated magnetofluorescent nanoparticles by strain‐promoted alkyne–nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2‐positive breast cancer cells.