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Topology‐Selective Chromatography Reveals Plasmid Supercoiling Shifts during Fermentation and Allows Rapid and Efficient Preparation of Topoisomers
Author(s) -
Mahut Marek,
Haller Elisabeth,
Ghazidezfuli Parisa,
Leitner Michael,
Ebner Andreas,
Hinterdorfer Peter,
Lindner Wolfgang,
Lämmerhofer Michael
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201106495
Subject(s) - dna supercoil , plasmid , fermentation , topology (electrical circuits) , dna , chemistry , chromatography , biology , biochemistry , mathematics , dna replication , combinatorics
Recognizing pDNA supercoils : Differently supercoiled species of the same plasmid DNA can be separated by topology‐selective chromatography. Two‐dimensional HPLC proved that the supercoiling changes during fermentation. Thus, a new quality criterium might help to optimize the effectivity of future genetic drugs and vaccines.