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Chemical Synthesis of Ubiquitinated Peptides with Varying Lengths and Types of Ubiquitin Chains to Explore the Activity of Deubiquitinases
Author(s) -
Bavikar Sudhir N.,
Spasser Liat,
HajYahya Mahmood,
Karthikeyan Subramanian Vedhanarayanan,
Moyal Tal,
Ajish Kumar K. S.,
Brik Ashraf
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201106430
Subject(s) - ubiquitin , lysine , peptide , deubiquitinating enzyme , chemistry , ubiquitin conjugating enzyme , enzyme , biochemistry , stereochemistry , amino acid , ubiquitin ligase , gene
Adding one at a time : A general and effective synthesis yields a peptide attached to mono‐, di‐, tri‐, and tetraubiquitin (Ub) chains (see picture for peptides with Ub and Ub 4 ), linked through lysine residues K48 or K63. These sets of ubiquitinated peptides were prepared in good quantities, and the activity of the enzymes UCH‐L3 and IsoT with these different substrates was studied.