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A FlAsH‐Based Cross‐Linker to Study Protein Interactions in Living Cells
Author(s) -
Rutkowska Anna,
Haering Christian H.,
Schultz Carsten
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201106404
Subject(s) - linker , in vitro , flash (photography) , covalent bond , peptide , derivative (finance) , chemistry , protein–protein interaction , amino acid , combinatorial chemistry , biophysics , biochemistry , biology , computer science , physics , organic chemistry , financial economics , economics , operating system , optics
As you like it : xCrAsH, a dimeric derivative of the arsenical compound FlAsH, enables the highly specific, covalent cross‐linking of two proteins containing a 12 amino acid peptide tag. This inducible and (by addition of dithiols) reversible system can be used to detect and manipulate protein–protein interactions both in vitro and in living cells (see picture).