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Solid‐State NMR Spectroscopy on Cellular Preparations Enhanced by Dynamic Nuclear Polarization
Author(s) -
Renault Marie,
Pawsey Shane,
Bos Martine P.,
Koers Eline J.,
Nand Deepak,
Tommassenvan Boxtel Ria,
Rosay Melanie,
Tommassen Jan,
Maas Werner E.,
Baldus Marc
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201105984
Subject(s) - polarization (electrochemistry) , solid state , nuclear magnetic resonance spectroscopy , spectroscopy , microwave , solid state nuclear magnetic resonance , chemistry , analytical chemistry (journal) , nuclear magnetic resonance , materials science , physics , computer science , telecommunications , chromatography , quantum mechanics
A peek inside : Dynamic nuclear polarization (DNP) enhances the spectroscopic sensitivity of solid‐state NMR measurements of uniformly ( 13 C, 15 N)‐labeled preparations of Escherichia coli cells by more than an order of magnitude (see picture; MW=microwaves, ε =enhancement factor). The major molecular components in the cells can be characterized in this way.