Premium
Enzymatic Total Synthesis of Defucogilvocarcin M and Its Implications for Gilvocarcin Biosynthesis
Author(s) -
Pahari Pallab,
Kharel Madan K.,
Shepherd Micah D.,
van Lanen Steven G.,
Rohr Jürgen
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201105882
Subject(s) - biosynthesis , enzyme , polyketide , chemistry , biochemistry , computational biology , computer science , stereochemistry , information retrieval , biology
Teamwork : Defucogilvocarcin M ( 1 , see scheme) was synthesized in a one‐pot, enzymatic reaction from acetyl‐CoA and malonyl‐CoA by a combination of 15 enzymes obtained from E. coli as well as the gilvocarcin, jadomycin, and ravidomycin biosynthetic pathways. The mixture of enzymes was systematically reduced and varied to further delineate the sequence of reactions in the complex, post‐polyketide steps of gilvocarcin biosynthesis.