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Multicolor Labeling of Living‐Virus Particles in Live Cells
Author(s) -
Zhou Peng,
Zheng Zhenhua,
Lu Wen,
Zhang Fuxian,
Zhang Zhenfeng,
Pang Daiwen,
Hu Bin,
He Zhike,
Wang Hanzhong
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201105701
Subject(s) - green fluorescent protein , infectivity , virus , nucleic acid , glycoprotein , fluorescence , viral replication , chemistry , biology , microbiology and biotechnology , virology , biophysics , biochemistry , gene , physics , quantum mechanics
Dual‐color virus particles were obtained by labeling a glycoprotein (GP64) on the surface of a baculovirus with a green fluorescent protein (GFP) and labeling the nucleic acid (dsDNA) at the same time with the red fluorescent [Ru(phen) 2 (dppz)] 2+ complex during the viral replication in host cells, by using an in vivo virus self‐assembly system. This labeling strategy does not affect the viral infectivity.