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Two‐Temperature Hybridization for Microarray Detection of Label‐Free MicroRNAs with Attomole Detection and Superior Specificity
Author(s) -
Lee Jeong Min,
Jung Yongwon
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201105605
Subject(s) - microrna , nucleic acid , microarray , computational biology , microbiology and biotechnology , chemistry , biology , gene expression , gene , genetics
Two is better than one : Two short locked nucleic acid based probes were used to collectively capture and detect microRNAs by a simple two‐temperature hybridization process. Intact microRNAs were directly measured down to attomolar concentrations with a high specificity and nearly four orders of magnitude of dynamic range. Single base mismatches in the microRNAs were potently discriminated from the perfectly matched targets.