Two‐Temperature Hybridization for Microarray Detection of Label‐Free MicroRNAs with Attomole Detection and Superior Specificity

Two is better than one : Two short locked nucleic acid based probes were used to collectively capture and detect microRNAs by a simple two‐temperature hybridization process. Intact microRNAs were directly measured down to attomolar concentrations with a high specificity and nearly four orders of magnitude of dynamic range. Single base mismatches in the microRNAs were potently discriminated from the perfectly matched targets.