Premium
Multivalent Glycocluster Design through Directed Evolution
Author(s) -
MacPherson Iain S.,
Temme J. Sebastian,
Habeshian Sevan,
Felczak Krzysztof,
Pankiewicz Krzysztof,
Hedstrom Lizbeth,
Krauss Isaac J.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201105555
Subject(s) - avidity , glycan , chemistry , dendrimer , ligand (biochemistry) , selectin , receptor , biochemistry , microbiology and biotechnology , protein–protein interaction , small molecule , computational biology , biophysics , biology , glycoprotein , cell adhesion molecule , antigen , genetics
Selection with modified aptamers : A method is described for design of glycocluster ligands by directed evolution. Glycan azides are attached to a library of alkyne‐containing DNA sequences. DNA sequences which cluster the glycans best for multivalent binding to the target are selected and amplified. This method has been applied to the development of high‐mannose clusters which bind HIV‐neutralizing antibody 2G12.