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Dynamics and Multiple Stable Binding Modes of DNA Intercalators Revealed by Single‐Molecule Force Spectroscopy
Author(s) -
Paik D. Hern,
Perkins Thomas T.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201105540
Subject(s) - force spectroscopy , intercalation (chemistry) , dna , molecule , spectroscopy , biophysics , chemistry , chemical physics , crystallography , physics , biology , biochemistry , quantum mechanics , inorganic chemistry , organic chemistry
Lights off, but someone is home : Single‐molecule force spectroscopy was used to investigate DNA intercalation in the presence and absence of unbound dye (see picture). Force‐induced intercalation, traditionally ascribed to dye from solution binding to the DNA, occured approximately two hours after the removal of the free dye. The results show that binding/unbinding and intercalation/deintercalation are distinct processes that occur on very different time scales.