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Back Cover: Tuning DNA Stability To Achieve Turnover in Template for an Enzymatic Ligation Reaction (Angew. Chem. Int. Ed. 38/2011)
Author(s) -
Kausar Abu,
McKay Rosalie D.,
Lam Jade,
Bhogal Rohan S.,
Tang Alexandra Y.,
GibbsDavis Julianne M.
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201104919
Subject(s) - dna ligase , ligation , dna ligases , template , dna , chemistry , loop mediated isothermal amplification , dna replication , biophysics , microbiology and biotechnology , materials science , biochemistry , biology , nanotechnology
Unlike native DNA a DNA template containing a destabilizing modification dissociates from the templated ligation product after its formation. As reported by J. M. Gibbs‐Davis et al. in their Communication on page 8922 ff., such destabilizing templates turn over in enzymatic ligation reactions, thereby leading to multiple strands per template. Consequently, isothermal DNA amplification and even replication can be achieved using ligase, simple destabilizing modifications, and standard DNA functional groups.