Premium
Insights into Neuronal Cell Metabolism Using NMR Spectroscopy: Uridyl Diphosphate N ‐Acetyl‐Glucosamine as a Unique Metabolic Marker
Author(s) -
Gallinger Anika,
Biet Thorsten,
Pellerin Luc,
Peters Thomas
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201104836
Subject(s) - glycolysis , flux (metallurgy) , metabolism , citric acid cycle , biochemistry , tricarboxylic acid , energy metabolism , nuclear magnetic resonance spectroscopy , metabolic pathway , metabolic flux analysis , cell , metabolic network , chemistry , computational biology , biology , stereochemistry , organic chemistry , endocrinology
Making the switch : Compounds 1 and 2 are used as metabolic markers for NMR detection. When neuronal cells switch to a glycolytic state, an uneven distribution of 13 C in the N‐acetyl group results, thus giving a mixture of the metabolites 1 and 2 . It is therefore possible to monitor flux through different metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, and the hexosamine biosynthetic pathway, using a single molecule.