Insights into Neuronal Cell Metabolism Using NMR Spectroscopy: Uridyl Diphosphate  N ‐Acetyl‐Glucosamine as a Unique Metabolic Marker | Zendy

Gallinger Anika | Zendy; Biet Thorsten | Zendy; Pellerin Luc | Zendy; Peters Thomas | Zendy

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Insights into Neuronal Cell Metabolism Using NMR Spectroscopy: Uridyl Diphosphate N ‐Acetyl‐Glucosamine as a Unique Metabolic Marker

Author(s) -

Gallinger Anika,

Biet Thorsten,

Pellerin Luc,

Peters Thomas

Publication year - 2011

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.201104836

Subject(s) - glycolysis , flux (metallurgy) , metabolism , citric acid cycle , biochemistry , tricarboxylic acid , energy metabolism , nuclear magnetic resonance spectroscopy , metabolic pathway , metabolic flux analysis , cell , metabolic network , chemistry , computational biology , biology , stereochemistry , organic chemistry , endocrinology

Making the switch : Compounds 1 and 2 are used as metabolic markers for NMR detection. When neuronal cells switch to a glycolytic state, an uneven distribution of 13 C in the N‐acetyl group results, thus giving a mixture of the metabolites 1 and 2 . It is therefore possible to monitor flux through different metabolic pathways, such as glycolysis, the tricarboxylic acid cycle, and the hexosamine biosynthetic pathway, using a single molecule.

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