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Segmental Isotopic Labeling of Ubiquitin Chains To Unravel Monomer‐Specific Molecular Behavior
Author(s) -
Castañeda Carlos A.,
Spasser Liat,
Bavikar Sudhir N.,
Brik Ashraf,
Fushman David
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201104649
Subject(s) - ubiquitins , ubiquitin , monomer , chemistry , ubiquitin ligase , ligand (biochemistry) , biochemistry , biophysics , polymer , biology , organic chemistry , receptor , gene
“Seeing” each monomer in ubiquitin chains : By combination of the chemical synthesis of one ubiquitin monomer with recombinant expression of its 15 N‐containing counterpart, the labeling of ubiquitins in non‐enzymatically assembled di‐ubiquitin chains was achieved, to enable monomer‐specific studies in solution. This allowed the first characterization of the structure, dynamics, intrachain interactions, and ligand binding of Lys33‐linked di‐ubiquitin.