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Fluorescence Detection of Intron Lariat RNA with Reduction‐Triggered Fluorescent Probes
Author(s) -
Furukawa Kazuhiro,
Abe Hiroshi,
Tamura Yasutsugu,
Yoshimoto Rei,
Yoshida Minoru,
Tsuneda Satoshi,
Ito Yoshihiro
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201104425
Subject(s) - fluorescence , fluorescein , rna , intron , chemistry , derivative (finance) , combinatorial chemistry , computational biology , biophysics , biochemistry , biology , gene , physics , quantum mechanics , financial economics , economics
Fluorescence through proximity : Reduction‐triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence‐specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.