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Formation of Sequence‐Independent Z‐DNA Induced by a Ruthenium Complex at Low Salt Concentrations
Author(s) -
Wu Zhiguo,
Tian Tian,
Yu Junping,
Weng Xiaocheng,
Liu Yi,
Zhou Xiang
Publication year - 2011
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201104422
Subject(s) - isothermal titration calorimetry , ruthenium , dna , salt (chemistry) , phenanthroline , sequence (biology) , aqueous solution , chemistry , titration , calorimetry , crystallography , stereochemistry , inorganic chemistry , biochemistry , physics , thermodynamics , catalysis
Switched : The metal complex [Ru(dip) 2 dppz] 2+ can induce the transition from B‐DNA to Z‐DNA and stabilize the Z conformation in aqueous low‐salt solution (see scheme; dip=4,7‐diphenyl‐1,10‐phenanthroline, dppz=dipyridophenazine). The transition is sequence‐independent (including non‐alternating pyrimidine–purine and AT‐rich sequences) and was monitored by CD and 2D NMR spectroscopy, AFM, isothermal titration calorimetry, and gel electrophoresis.