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Prediction of a Ligand‐Binding Niche within a Human Olfactory Receptor by Combining Site‐Directed Mutagenesis with Dynamic Homology Modeling
Author(s) -
Gelis Lian,
Wolf Steffen,
Hatt Hanns,
Neuhaus Eva M.,
Gerwert Klaus
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201103980
Subject(s) - computational biology , olfactory receptor , reprogramming , niche , homology modeling , biology , mutagenesis , binding site , mutant , site directed mutagenesis , homology (biology) , receptor , evolutionary biology , genetics , neuroscience , computer science , gene , ecology , biochemistry , enzyme
Reprogramming a smell receptor : The ligand‐binding niche within a three‐dimensional model of a human olfactory receptor has been predicted by dynamic homology modeling and confirmed experimentally by functional studies of site‐directed receptor mutants. Even a proposed reprogramming of the receptor's binding and activation properties was experimentally confirmed.